Crystallography Research Today is a free monthly online journal that collates and summarizes the latest research about Crystallography, including details on x-ray crystals, techniques, analyses, structures.

Solution-stirring method improves crystal quality of human triosephosphate isomerase.

Adachi H, Niino A, Kinoshita T, Warizaya M, Maruki R, Takano K, Matsumura H, Inoue T, Murakami S, Mori Y, Sasaki T

SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 A resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 A. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity.

Published 28 February 2006 in J Biosci Bioeng, 101(1): 83-6.
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